| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
 dna Videos | |
| |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
03-20-2007, 12:20 PM
| |||||
| |||||
 Explain the process of electrophoresis gel and... ...how it is used to seperate DNA fragments? Explain the process of electrophoresis gel and how it is used to seperate DNA fragments        |
| Today
| ||||
| ||||
 Sponsored Links |
|
03-20-2007, 12:40 PM
| |||||
| |||||
| the gel is like a matrix which offers reistance to the fragments of dna. the dna has polarity which means it will be effected by electric current. when the electricity passes through the gel and the wells with the sample, it picks up bits of the dna and carries it toward the other end of the gel. smaller pieces hit less resistance from the gel and travel quicky. larger pieces hit more resitance and travel slowly. usually there will be a control or a ladder that will travel along at the same time. you can compare the distance your dna fragments travel to the ladder and you'll know aproximately how many base pairs are in each band. |
|
| Thread Tools | |
| Display Modes | |
|
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Agarose gel electrophoresis of PCR products - Method and Protocol | domba | PCR - Polymerase Chain Reaction Forum | 4 | 05-23-2008 04:42 PM |
| During electrophoresis, gels that are run... | ralph g | DNA Techniques | 1 | 12-17-2007 06:22 PM |
| TBE vs TAE for Gel Electrophoresis | dnamolecule | Basic Lab Protocols and Techniques | 5 | 11-15-2007 05:27 AM |
| briefly explain the process of electrophoresis and... | micky | DNA Techniques | 1 | 03-19-2007 11:53 PM |
| RNA electrophoresis | admin | Article Discussion | 0 | 03-17-2007 04:44 AM |
Linear Mode
