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What Are The Functions Of Sds, Edta And Chloroform

What Are The Functions Of Sds, Edta And Chloroform - DNA Techniques

What Are The Functions Of Sds, Edta And Chloroform - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 03-20-2007, 08:22 AM
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Default What Are The Functions Of Sds, Edta And Chloroform



IN DNA ISOLATION?what is the function of quanidine thiocyan
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Old 03-20-2007, 11:24 AM
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as far as my knowledge goes SDS is a deterget it helps in distroying the proteins in the sample,EDTA is a chelator which chelates the ions lik Ca+ and Mg[req by enzym to break DNA],and chloroform being organic solvent keeps the DNA away frm the water and prevts it frm getting dissolved
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Old 10-06-2009, 05:52 AM
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Default Re: What Are The Functions Of Sds, Edta And Chloroform

function of chloroform in RT PCR
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Old 12-07-2009, 11:04 AM
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Default Re: What Are The Functions Of Sds, Edta And Chloroform

Quote:
Originally Posted by jjaiganesh View Post
function of chloroform in RT PCR
SDS is the most common dissociating agent used to denature native proteins to individual polypeptides.
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