Is Ecoli JM110 strain is methyl deficient

[sailesh_malla]

strain? Can we use the plasmid DNA from this strain to streptomyces?

[patatonic]

The JM110 strain is useful for preparing plasmid or phagemid DNA that is free of Dam or Dcm methylation so that the DNA can be restriction digested by one or more methylation-sensitive restriction enzymes.

JM110 Genotype: rpsL (Strr) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 ?(lac-proAB) [F´ traD36 proAB lacIqZ?M15]. (Genes listed signify mutant alleles. Genes on the F´ episome, however, are wild-type unless indicated otherwise.)

JM110 is deficient for two methylases (Dam and Dcm) found in most strains of E. coli. Dam methylase recognizes the DNA sequence GATC and methylates the adenine residue at the N-6 position, while Dcm methylase recognizes the DNA sequence CCAGG and CCTGG and methylates the internal cytosine at the C-5 position. JM110 cells contain the lacIqZ?M15 gene on the F´ episome, allowing blue-white
screening for recombinant plasmids.

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