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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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03-18-2007, 10:07 AM
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 How many bands will appear on the agarose gel if the... How many bands will appear on an agarose gel if the DNA sequence is treated with HaeIII? cheers! Last edited by admin; 03-18-2007 at 10:32 AM.        |
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03-18-2007, 10:33 AM
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| Re: How many bands will appear on the agarose gel if the... Hello Andrea, welcome to the forums. We need a bit more information. What DNA sequence are you trying to cut? You can analyze your sequence with Web Cutter.  |
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03-18-2007, 05:30 PM
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| HaeIII recognizes the following sequence: 5'...GG/CC...3' 3'...CC/GG...5' If your DNA is short and linear, the number of band will be one plus the number of times the above sequence appears (assuming your enzymatic cleaveage reaction worked properly). If your DNA is circular, you will get the same number of bands as you have instances of the above sequence. (usually the band due to circular DNA shows up elsewhere) Also, look at your bp ladder control lane to get an idea of size of the fragments, and try to correlate that with the expect cuts based on your DNA sequence. |
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