|Register||Search||Today's Posts||Mark Forums Read|
|DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.|
| ||LinkBack||Thread Tools||Display Modes|
Synthetic DNA Quantifications using Nanodrop
I'm wondering if anyone can give me advice on DNA quantification using the nanodrop. I've ordered ssDNA oligos from sigma, which comes with a recipe for obtaining 100uM concentrations. However when I was told to quantify it using the nanodrop, it gave weird results, measuring up to 130-150uM. I initially computed for the concentration by using the "ng/uL" reading and dividing it my its molecular weight and the appropriate volume to get the concentration in uM.
However I've recently realized that I can also get the A260 from the raw data and divide that value by the epsilon of the oligos. I tried this for the oligos we have now, and they give slightly less than what they should.
My question is whether or not I can rely on the default nanodrop settings to quantify my DNA.
|dna , nanodrop , quantifications , synthetic|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Tell me about using a Nanodrop for protein quantitation||Bonnie||Protein Science||5||06-18-2010 10:46 AM|
|Post Doctoral Positions||Medford,June||Arabidopsis and Plant Biology||0||10-22-2009 03:42 PM|
|Plant Synthetic Biology Post Doctoral Positions||Medford,June||Arabidopsis and Plant Biology||0||02-04-2009 10:18 PM|
|The Second International Conference on Synthetic Biology: Understanding & Perception||moleculardude||Molecular Biology Lectures and Videos||0||06-11-2007 12:30 PM|
|Question Difference Between Synthetic and Organic Hormonesfirstname.lastname@example.org||Organic Chemistry Forum||0||08-25-2006 08:21 AM|