Synthetic DNA Quantifications using Nanodrop

[evilhamster]

Hi everyone,

I'm wondering if anyone can give me advice on DNA quantification using the nanodrop. I've ordered ssDNA oligos from sigma, which comes with a recipe for obtaining 100uM concentrations. However when I was told to quantify it using the nanodrop, it gave weird results, measuring up to 130-150uM. I initially computed for the concentration by using the "ng/uL" reading and dividing it my its molecular weight and the appropriate volume to get the concentration in uM.

However I've recently realized that I can also get the A260 from the raw data and divide that value by the epsilon of the oligos. I tried this for the oligos we have now, and they give slightly less than what they should.

My question is whether or not I can rely on the default nanodrop settings to quantify my DNA.


Thanks,
evilhamster