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Synthetic DNA Quantifications using Nanodrop

Synthetic DNA Quantifications using Nanodrop - DNA Forum

Synthetic DNA Quantifications using Nanodrop - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 10-05-2012, 12:55 PM
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Default Synthetic DNA Quantifications using Nanodrop



Hi everyone,

I'm wondering if anyone can give me advice on DNA quantification using the nanodrop. I've ordered ssDNA oligos from sigma, which comes with a recipe for obtaining 100uM concentrations. However when I was told to quantify it using the nanodrop, it gave weird results, measuring up to 130-150uM. I initially computed for the concentration by using the "ng/uL" reading and dividing it my its molecular weight and the appropriate volume to get the concentration in uM.

However I've recently realized that I can also get the A260 from the raw data and divide that value by the epsilon of the oligos. I tried this for the oligos we have now, and they give slightly less than what they should.

My question is whether or not I can rely on the default nanodrop settings to quantify my DNA.


Thanks,
evilhamster
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