Synthetic DNA Quantifications using Nanodrop
I'm wondering if anyone can give me advice on DNA quantification using the nanodrop. I've ordered ssDNA oligos from sigma, which comes with a recipe for obtaining 100uM concentrations. However when I was told to quantify it using the nanodrop, it gave weird results, measuring up to 130-150uM. I initially computed for the concentration by using the "ng/uL" reading and dividing it my its molecular weight and the appropriate volume to get the concentration in uM.
However I've recently realized that I can also get the A260 from the raw data and divide that value by the epsilon of the oligos. I tried this for the oligos we have now, and they give slightly less than what they should.
My question is whether or not I can rely on the default nanodrop settings to quantify my DNA.
|All times are GMT. The time now is 07:42 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2013, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved