This might be a dumb question, but I'm trying to teach myself all of this.
Is there a different way in determining the estimated concentration when using Cts versus RFUs?
Currently, I am determining the concentration of a sample, using Cts, by getting the difference between the Ct and the y-intercept, and then dividing that by the slope of the standard curve.
Could I use this same formula to determine the concentration, using RFUs? All of the data for the standard curve is also in RFUs.