Estimating Concentration

[boblee1980]

Hello,

This might be a dumb question, but I'm trying to teach myself all of this.

Is there a different way in determining the estimated concentration when using Cts versus RFUs?

Currently, I am determining the concentration of a sample, using Cts, by getting the difference between the Ct and the y-intercept, and then dividing that by the slope of the standard curve.

Could I use this same formula to determine the concentration, using RFUs? All of the data for the standard curve is also in RFUs.

Thanks,
Bob

[boblee1980]

I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.

Thanks,

[boblee1980]

I think I found an answer to my own question. Since both data analysis methods use the a regression line due to the concentrations and the samples being linearly correlated; I use the slope and intercept to back calculate the concentration from both CT and RFU values. Is this correct?

[csxw]

I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.