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Estimating Concentration

Estimating Concentration - DNA Forum

Estimating Concentration - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 04-04-2012, 07:29 PM
Pipette Filler
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Default Estimating Concentration



Hello,

This might be a dumb question, but I'm trying to teach myself all of this.

Is there a different way in determining the estimated concentration when using Cts versus RFUs?

Currently, I am determining the concentration of a sample, using Cts, by getting the difference between the Ct and the y-intercept, and then dividing that by the slope of the standard curve.

Could I use this same formula to determine the concentration, using RFUs? All of the data for the standard curve is also in RFUs.

Thanks,
Bob
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Old 04-04-2012, 07:38 PM
Pipette Filler
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Smile Re: Estimating Concentration

I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.

Thanks,
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Old 04-04-2012, 08:49 PM
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Default Re: Estimating Concentration

I think I found an answer to my own question. Since both data analysis methods use the a regression line due to the concentrations and the samples being linearly correlated; I use the slope and intercept to back calculate the concentration from both CT and RFU values. Is this correct?
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Old 06-02-2012, 01:31 AM
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Default Re: Estimating Concentration

I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.
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conc , concentration , estimating , qpcr , quantitation , rfu


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