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boblee1980 04-04-2012 07:29 PM

Estimating Concentration
 
Hello,

This might be a dumb question, but I'm trying to teach myself all of this.

Is there a different way in determining the estimated concentration when using Cts versus RFUs?

Currently, I am determining the concentration of a sample, using Cts, by getting the difference between the Ct and the y-intercept, and then dividing that by the slope of the standard curve.

Could I use this same formula to determine the concentration, using RFUs? All of the data for the standard curve is also in RFUs.

Thanks,
Bob

boblee1980 04-04-2012 07:38 PM

Re: Estimating Concentration
 
I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.

Thanks,

boblee1980 04-04-2012 08:49 PM

Re: Estimating Concentration
 
I think I found an answer to my own question. Since both data analysis methods use the a regression line due to the concentrations and the samples being linearly correlated; I use the slope and intercept to back calculate the concentration from both CT and RFU values. Is this correct?

csxw 06-02-2012 01:31 AM

Re: Estimating Concentration
 
I forgot to mention, I'm using the Invitrogen RNA Quant-iT Assay Kit.


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