Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


ChIP Sonication

ChIP Sonication - DNA Forum

ChIP Sonication - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-16-2011, 08:37 AM
Pipette Filler
Points: 31, Level: 1 Points: 31, Level: 1 Points: 31, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default ChIP Sonication



Hi all,

I'm trying to optimize sonication as part of my ChIP protocol. Instead of seeing a smear after sonication, I consistently see a single band (perhaps spread over only 200bp) sometimes at 200-400bp and once at 600-800bp. I just did a test to check the chromatin before sonication, and then after every burst, and interestingly I found that before sonication my DNA is only 300-400bp, and sonication (up to 10 bursts) has made no difference. Any suggestions??

I've uploaded a pic of the chromatin before sonication, and then after 1 burst, 2 bursts, etc.
Attached Images
File Type: jpg c2c12 sonication 16-12-11.jpg (6.3 KB, 9 views)
Reply With Quote
  #2  
Old 01-03-2013, 05:53 AM
Pipette Filler
Points: 93, Level: 1 Points: 93, Level: 1 Points: 93, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2012
Posts: 6
Thanks: 2
Thanked 0 Times in 0 Posts
Default Re: ChIP Sonication

Is your problem solved? I have the similar problem now? I cannot get smeared bands. Could you give me some suggestions? Thanks!
Reply With Quote
  #3  
Old 01-04-2013, 08:32 PM
Pipette Filler
Points: 93, Level: 1 Points: 93, Level: 1 Points: 93, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2012
Posts: 6
Thanks: 2
Thanked 0 Times in 0 Posts
Default Re: ChIP Sonication

Hi, is your problem solved? It seems like your gel didn't run long enough. Why not to run a gel much longer and with low power?
Reply With Quote
  #4  
Old 01-11-2013, 01:12 PM
Volunteer
Points: 131, Level: 2 Points: 131, Level: 2 Points: 131, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 32
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー

[

[

[
Reply With Quote
  #5  
Old 01-11-2013, 01:14 PM
Volunteer
Points: 131, Level: 2 Points: 131, Level: 2 Points: 131, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 32
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー 腕時計

[

[

[
Reply With Quote
  #6  
Old 01-11-2013, 01:19 PM
Volunteer
Points: 131, Level: 2 Points: 131, Level: 2 Points: 131, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 32
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー 時計

[

[

[
Reply With Quote
  #7  
Old 01-11-2013, 06:01 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー 激安

[

[

[
Reply With Quote
  #8  
Old 01-11-2013, 06:24 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー

[

[

[
Reply With Quote
  #9  
Old 01-11-2013, 06:46 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー コート

[

[

[
Reply With Quote
  #10  
Old 01-11-2013, 06:49 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー 腕時計

[

[

[
Reply With Quote
Reply

Tags
chip , sonication


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nanotech: La nuova retina un chip fotovoltaico \(`.Emael.\) Forum Biologia 1 10-19-2012 02:55 AM
ChIP sonication fragments too small jchang ChIP Chromatin Immunoprecipitation Forum 2 02-14-2012 07:56 PM
ChIP Assay Sonication Problems IoDiNE ChIP Chromatin Immunoprecipitation Forum 3 02-17-2009 02:12 PM
ChIP on chip Affymetrix deebee12 Microarrays Forum 0 07-25-2008 12:26 PM
Chip sotto pelle, crescono i fan negli Usa \(`.Emael.\) Forum Biologia 1 01-10-2006 06:10 PM


All times are GMT. The time now is 12:55 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15793 seconds with 17 queries