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-   -   ChIP Sonication (http://www.molecularstation.com/forum/dna-forum/84319-chip-sonication.html)

JHulin 12-16-2011 08:37 AM

ChIP Sonication
 
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Hi all,

I'm trying to optimize sonication as part of my ChIP protocol. Instead of seeing a smear after sonication, I consistently see a single band (perhaps spread over only 200bp) sometimes at 200-400bp and once at 600-800bp. I just did a test to check the chromatin before sonication, and then after every burst, and interestingly I found that before sonication my DNA is only 300-400bp, and sonication (up to 10 bursts) has made no difference. Any suggestions??

I've uploaded a pic of the chromatin before sonication, and then after 1 burst, 2 bursts, etc.

ChIP Kick 01-03-2013 05:53 AM

Re: ChIP Sonication
 
Is your problem solved? I have the similar problem now? I cannot get smeared bands. Could you give me some suggestions? Thanks!

ChIP Kick 01-04-2013 08:32 PM

Re: ChIP Sonication
 
Hi, is your problem solved? It seems like your gel didn't run long enough. Why not to run a gel much longer and with low power?

Georgialgr 01-11-2013 01:12 PM

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Georgialgr 01-11-2013 01:14 PM

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Georgialgr 01-11-2013 01:19 PM

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Jenniferayt 01-11-2013 06:01 PM

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Jenniferayt 01-11-2013 06:24 PM

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Jenniferayt 01-11-2013 06:46 PM

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Jenniferayt 01-11-2013 06:49 PM

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