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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


RAPD-PCR Problem...

RAPD-PCR Problem... - DNA Forum

RAPD-PCR Problem... - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 11-10-2011, 06:07 AM
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Default RAPD-PCR Problem...



Dear All,
I would like to ask my problem about RAPD-PCR.
For RAPD-PCR i am using two primers M13 and RAPD-2.
The same genomic DNA is not going to be amplified by both the primers.
I have 10 genomic DNA samples, of which 3 genomic DNA samples are amplified
by the both the above mentioned primers. 4 genomic DNA samples are amplified
by the M13 but not by RAPD-2 and 2 genomic DNA samples are amplified by the RAPD-2 but not by M13 primer.

Kindly help me how to sort out these problems.

A. K. Dubey, PhD Student
Pondicherry University, Puducherry.
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