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measuring DNA Fragments after Benzonase
Currently I am doing protein purification utilizing Benzonase to remove or degrade DNA in my preps. One of the standards I must meet besides total ng content is that fragments must be below 200bp.
So from each of the stages of protein purification I take a sample and do a QIAamp DNA clean up/extraction and run the samples out on a 2% agarose gel. Now of course since I use Benzonase/nuclease I get a lot of streaking with a brighter high density section in each lane.
Is there anyway I can establish what my highest bp size fragment is within the agarose gel streak or could someone recommend an alternative technique to establish what my largest fragment size would be?
|benzonase , dna , fragments , measuring|
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