Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Buffy coat

Buffy coat - DNA Forum

Buffy coat - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-19-2011, 04:26 PM
Pipette Filler
Points: 295, Level: 5 Points: 295, Level: 5 Points: 295, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2011
Posts: 19
Thanks: 0
Thanked 2 Times in 2 Posts
Default Buffy coat



Hello all,

I'm working with whole blood and isolating plasma/buffy coat from EDTA treated tubes. I'm centrifuging the samples to isolate the layers based on density and pulling the plasma and buffy coat respectively. From here I will be pelleting the buffy coat and storing the pellets and plasma in -80 for later DNA/RNA extractions. I am using the Fujifilm autogen to carry out extractions. I am curious how many extractions I will be able to carry out using a single buffy coat pellet. The required cell count for a single sample prep is 3 x 10^6 suspended in 200 micro-liters pbs. Any help or suggetions would be greatly appreciated.

thanks and cheers,

Matthew
Reply With Quote
Reply

Tags
buffy , buffy coat , coat , dna , isolation , plasma , rna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Buffy Coat Cell Perparation Fred Protocols and Methods Forum 1 09-20-2004 12:45 AM
Buffy Coat Cell Perparation Fred Protocols and Methods Forum 0 08-25-2004 10:29 PM
Isolation of buffy coat (WBC) from whole blood (only 500 Ál) after removing the plasma Elisabet W Protocols and Methods Forum 0 03-02-2004 12:48 PM
Tool Coat Precursors Bala Chemistry Forum 4 10-29-2003 05:51 PM
RNA isolation from frozen buffy coat Anita Protocols and Methods Forum 0 07-03-2003 01:01 PM


All times are GMT. The time now is 07:55 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11113 seconds with 16 queries