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| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
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#1
07-19-2011, 04:26 PM
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 Buffy coat Hello all, I'm working with whole blood and isolating plasma/buffy coat from EDTA treated tubes. I'm centrifuging the samples to isolate the layers based on density and pulling the plasma and buffy coat respectively. From here I will be pelleting the buffy coat and storing the pellets and plasma in -80 for later DNA/RNA extractions. I am using the Fujifilm autogen to carry out extractions. I am curious how many extractions I will be able to carry out using a single buffy coat pellet. The required cell count for a single sample prep is 3 x 10^6 suspended in 200 micro-liters pbs. Any help or suggetions would be greatly appreciated. thanks and cheers, Matthew  |
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| buffy , buffy coat , coat , dna , isolation , plasma , rna |
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