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TAE buffer

TAE buffer - DNA Forum

TAE buffer - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 07-17-2011, 09:38 PM
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Default TAE buffer



Basic question, of which I cannot find the answer on the web: What are the functions of the components of TAE buffer, when using it as a running buffer during agarose DNA gel electrophoresis?

Thank you in advance.
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Old 07-19-2011, 02:03 AM
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Default Re: TAE buffer

Correct me if I'm wrong: Tris is a pH buffering substance, EDTA chelates metallic ions to keep DNAses unactive and Acetic acid donate ions so electric current can pass through.
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Old 07-20-2011, 09:07 PM
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Default Re: TAE buffer

Thanks for your reply.

I know Tris and EDTA are used in TE buffer to dissolve DNA, and in that buffer you need both pH8 and the chelating of divalent kations to inactivate DNases.

However, I was wondering whether EDTA would serve that same purpose in running buffer. Would DNases really be capable of degrading the DNA within the context of a solid agarosegel while there is current, within the timeframe of ~1h (the average time I run a gel)? Or does the EDTA has a different function in TAE as compared to its funtion in TE buffer?
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Old 07-21-2011, 05:17 AM
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Default Re: TAE buffer

I actually don't know about that. It's a good question worth checking Molecular Cloning again. If I find something there or elsewhere I'll let you know. Also, let me know if you find something about it.
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Old 09-04-2011, 09:26 AM
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Default Re: TAE buffer

Quote:
Originally Posted by luisillo View Post
Correct me if I'm wrong: Tris is a pH buffering substance, EDTA chelates metallic ions to keep DNAses unactive and Acetic acid donate ions so electric current can pass through.


Useful and best post!
I'm searching for some answer about this.
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