| | Re: TAE buffer
Thanks for your reply.
I know Tris and EDTA are used in TE buffer to dissolve DNA, and in that buffer you need both pH8 and the chelating of divalent kations to inactivate DNases.
However, I was wondering whether EDTA would serve that same purpose in running buffer. Would DNases really be capable of degrading the DNA within the context of a solid agarosegel while there is current, within the timeframe of ~1h (the average time I run a gel)? Or does the EDTA has a different function in TAE as compared to its funtion in TE buffer?