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#1
07-09-2011, 05:15 AM
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 Primer specificity Dear All, Hello! I searched for an 18S rRNA gene of an organism on the NCBI website. I found the sequence and designed primers using the primer tool on the same website. Now, I want to check the primer specificity (i.e. can I use this primer as an identification tool to detect a specific species using PCR) and the question is: do I need to BLAST the primer sequence (both forward and reverse) or to BLAST the sequence of the amplified fragment (PCR product). Many thanks in advance. Best regards, Mohammad  |
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#2
07-21-2011, 07:21 PM
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| Re: Primer specificity Check your primers using in silico PCR: [Only registered users see links. ] blasting the primer sequences is never bad.. |
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