I had a question about the quantification of DNA using the Nanodrop,
I amplified my DNA using normal PCR amplification i purified it with a Qiaquick PCR purification kit and then i measured my samples using the nanodrop, i had the following results:
Concentration (ng/ul) = 18,0
260/280 nm = 1,78
260/230 nm = 2,88!! <- This value concerns me
Does anyone of you have an idea what this high value means, i have looked up some forums and they said it was because the concentration of the DNA was too low but i don't think that this is the problem
Can anyone help me?