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|DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.|
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DNA denaturation and hyperchromicity
did somebody measure UV of DNA at 260 nm before and after denaturation?
As I know, after denaturation absorbance should be higher in 30-40%. But in my experiment I observed only 10% increase after thermal denaturation. I do not know if I do something wrong. Please, help.
|denaturation , dna , hyperchromicity|