| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| Hi all, did somebody measure UV of DNA at 260 nm before and after denaturation? As I know, after denaturation absorbance should be higher in 30-40%. But in my experiment I observed only 10% increase after thermal denaturation. I do not know if I do something wrong. Please, help. Thanks. Adriana |
| Tags |
| denaturation , dna , hyperchromicity |
| Thread Tools | |
| Display Modes | |
|
|