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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


DNA denaturation and hyperchromicity

DNA denaturation and hyperchromicity - DNA Forum

DNA denaturation and hyperchromicity - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 04-21-2011, 06:44 AM
Pipette Filler
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Default DNA denaturation and hyperchromicity



Hi all,
did somebody measure UV of DNA at 260 nm before and after denaturation?
As I know, after denaturation absorbance should be higher in 30-40%. But in my experiment I observed only 10% increase after thermal denaturation. I do not know if I do something wrong. Please, help.
Thanks. Adriana
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denaturation , dna , hyperchromicity


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