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-   -   DNA denaturation and hyperchromicity (http://www.molecularstation.com/forum/dna-forum/82391-dna-denaturation-hyperchromicity.html)

altair155 04-21-2011 06:44 AM

DNA denaturation and hyperchromicity
 
Hi all,
did somebody measure UV of DNA at 260 nm before and after denaturation?
As I know, after denaturation absorbance should be higher in 30-40%. But in my experiment I observed only 10% increase after thermal denaturation. I do not know if I do something wrong. Please, help.
Thanks. Adriana


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