Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


HELP HELP HELP HELP with pBABE

HELP HELP HELP HELP with pBABE - DNA Forum

HELP HELP HELP HELP with pBABE - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-09-2011, 04:23 PM
Pipette Filler
Points: 358, Level: 7 Points: 358, Level: 7 Points: 358, Level: 7
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2011
Location: SF
Posts: 19
Thanks: 10
Thanked 5 Times in 5 Posts
Unhappy HELP HELP HELP HELP with pBABE



Hi Guys,

I recently switched from a cozy DNA lab into a completely foreign territory of protein based cell culture lab. I came into a lab that has dwindling stalks of its most precious protein and I alone have to find a way to increase and replenish their stalks.

Anyway enough of that.

So they have cloned the 777 sequence (full length) into pBABE. pBabe if I understand correctly from what I have read is a retrovirus?

So my thought processes was that I would take this pBabe grow it up expand it ( is that possible?) and then infect empty phoenix vectors with it. After this is occurred we go ahead an infect the target cells using the Nolen lab protocol.

My Question to you guys is.
Does this make any sense logically.
How do I grow up the pBabe + insert(?) Whats the media whats the protocol?
IS the next step infection of the phenoix cells?
How can I save the grow up pBabe, so I don't lose reserves of that.

Please if anyone could help that would be greatly apprciated, or point in the right direction!!
Reply With Quote
  #2  
Old 03-09-2011, 04:51 PM
Pipette Filler
Points: 358, Level: 7 Points: 358, Level: 7 Points: 358, Level: 7
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2011
Location: SF
Posts: 19
Thanks: 10
Thanked 5 Times in 5 Posts
Default Re: HELP HELP HELP HELP with pBABE

I have also read that the way to get most virus transfected was pBabe(plus insert) + EEV into Bosc23 Cells then use these to target host cells. Anyone know how? Does this mean phoenix cells aren't even a viable choice?
Reply With Quote
Reply

Tags
cell culture , pbabe , phenoix cells , retrovirus


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 08:42 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12273 seconds with 15 queries