Hello every body I'm new for this forum I wish from the members who know solusion for the following question to answer :
Q/ A team of investigators carried out the following experiment :
oriC-containing , superhelical plasmid DNA was incubated at (37C) in a reaction mixture containing the proteins dnaA,dnaC,dnaB,Hu,SSB(single stranded DNA binding protein) and gyrase . the reaction also included ATP. At the end of incubation , the reaction mixture was deproteinized by the use of a detergent and a protease . Following deproteinization the DNA was subjected to electrophoresis in agarose gels . It has been observed that the DNA now migrates faster than the original plasmid DNA .
a) Identify the structure of the newly obtained DNA . How would you demonstrate that your identification is correct ?
b) What is the role of the proteins involved in generating this new DNA structure ?
And thank you for everything . please try to reply me quickly if possible.