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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


DNA Questions

DNA Questions - DNA Forum

DNA Questions - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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  #1  
Old 01-12-2011, 05:38 PM
Pipette Filler
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Default DNA Questions



Hello every body I'm new for this forum I wish from the members who know solusion for the following question to answer :

Q/ A team of investigators carried out the following experiment :
oriC-containing , superhelical plasmid DNA was incubated at (37C) in a reaction mixture containing the proteins dnaA,dnaC,dnaB,Hu,SSB(single stranded DNA binding protein) and gyrase . the reaction also included ATP. At the end of incubation , the reaction mixture was deproteinized by the use of a detergent and a protease . Following deproteinization the DNA was subjected to electrophoresis in agarose gels . It has been observed that the DNA now migrates faster than the original plasmid DNA .

a) Identify the structure of the newly obtained DNA . How would you demonstrate that your identification is correct ?
b) What is the role of the proteins involved in generating this new DNA structure ?


And thank you for everything . please try to reply me quickly if possible.

Last edited by admin; 01-13-2011 at 08:05 AM.
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  #2  
Old 01-13-2011, 05:43 AM
Pipette Filler
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Default Re: i want answer

good morning ...
Is there any answer please because i have an exam after 2 days
thank you..
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  #3  
Old 03-01-2011, 11:20 AM
Pipette Filler
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Default Re: DNA Questions

Hello Experts,

Can anyone tell me about DNA Methyl transferase 3B? I have no idea about this so i need your help.

Regards,
Stephen Jones
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