| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
12-22-2010, 09:14 PM
| |||||||||||
| |||||||||||
 Double Digestion Problem!!! I have (at least I think I have) modified my BAC. The problem that i am having is that when i pcr out a segment of my vector from the BAC I get a band that is exactly where it shoud be (forward primer is 5' of insert and reverse primer is within my insert). When I double digest (Sal1 and Not1) I dont see the appropriate shifts that I expect which tells me that my shuttle vector did not get inserted into my BAC?? My BAC is 190kb and I run the gel using pulse field electro. Any suggestions as to what is going on??? Thanks  |
|
| Tags |
| digestion , double , problem |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Problem with ligaition of a small fragment | thilsam | Molecular Cloning Forum | 1 | 09-18-2009 02:38 AM |
| In Deep Discussion: Double Aldol Condensation | Momer | Chemistry Forum | 0 | 05-27-2009 05:41 PM |
| Plasmid Digestion Problem | Haydee Lopez | Protocols and Methods Forum | 1 | 03-17-2009 11:27 PM |
| HELP: Need help solving a problem | Pat | Physics Forum | 4 | 04-19-2004 08:33 PM |
Linear Mode
