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weetree 10-30-2010 09:25 PM

Binary Vectors for Agrobacterium-Mediated Gene Transfer
 
I have been looking for a binary vector and have only found pGreen and pCLEAN. After looking into both of these vectors, I decided pCLEAN would be the best for me. So right now I am trying to figure out which vectors I want and came across some interesting information.

In a article evaluating pCLEAN vectors I saw that trans-genes located out side the T-DNA region (LB and RB borders) were being integrated into plant cell DNA.
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How does this occur, and does it occur naturally with a regular Ti-plasmid?

While you are viewing this, what vectors would you recommend I use? I am trying to engineer a plant expressing GFP for strictly visual representation of Agrobacterium-mediated gene transfer. I know what genes I want (GFP, HPT and Kan-R/Tet-R), but for the pCLEAN-S plasmids I am confused on why some of them contain T-DNA. What benefit is it to have two T-DNAs? In addition, why do some of the vectors use two LB sites?


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