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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


DNA silverstaining

DNA silverstaining - DNA Forum

DNA silverstaining - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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  #1  
Old 08-28-2010, 06:39 AM
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Default DNA silverstaining



Does anbody know about the logic behind the DNA silverstaining?
For example Why the band develop in developer?what kind of chemical reactions happen during developing?
Why we use cold water in developing stage or why we 've got to do developing stage in cold room?
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Old 08-30-2010, 06:22 PM
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Default Re: DNA silverstaining

Check this article:
Sanguinetti CJ, Dias-Neto E, Simpson AJ. Rapid silver staining and recovery of PCR products separated on polyacrilamide gels. Biotechniques 1994;17:914-921

I silver stained DNA in polyacrilamide gels for 3 years and never used anything cold. We did it at room temp and it worked well. The develop took 10 or so minutes and, from my experience, it worked better when the developing solution (3% NaOH, 0.5% formaldehide) was at RT.
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Old 09-23-2010, 06:17 AM
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Default Re: DNA silverstaining

Highly sensitive detection of nucleic acids in the nanogram range has been achieved by the specific chemical reduction of silver ions. The methods for silver-staining nucleic acids employ either a histologically derived procedure that uses ammoniacal solutions of silver , or a photochemically derived reaction in which silver binds to nucleic acid bases and is then selectively reduced by chemical agents or light. These silver staining protocols can be as sensitive as radioisotopic methods. However, they are complex and time consuming and require the preparation and handling of several solutions. In an attempt to simplify the routine use of silver stains to detect nucleic acids, BASSAM e] have optimized the photochemically derived silver stain .
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Old 11-04-2010, 07:36 PM
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Default Re: DNA silverstaining

Do you have an quick protocol for your RT stain?
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Old 11-08-2010, 07:05 PM
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Default Re: DNA silverstaining

Hi MEDIBENA. I guess you are asking that to me. The protocol is based on the article I mentioned before. I do have the protocol but is not that quick: you need around 40 min for the bands to develop and the handling of 3 different solutions (two can be stored for around a week and one must be prepared freshly).

Those are the cons. If you're still interested let me know and I'll post the protocol.
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Old 11-16-2010, 11:50 AM
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Default Re: DNA silverstaining

Those are the cons. If you're still interested let me know and I'll post the protocol.[/QUOTE]

thanks, I thought about quick protocol for a 40 min one I have a protocol too, Quick would be interesting but I gues there is with silver not such an option.
thanks peter
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Old 11-16-2010, 07:48 PM
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Default Re: DNA silverstaining

Guess WE have to develop something quicker or wait for someone to do it. Would be a nice patent, don't you think.
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Old 11-17-2010, 07:59 AM
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Default Re: DNA silverstaining

I would say so but i think with silver there are no shortcuts anymore
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