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Competition between haripin folding and primers binding of DNA in a (q)PCR
In a PCR after the denaturation step (forming single stranded DNA), DNA will have to possiblilty to bind it's own's nucleotides to it's own strain, forming hairpins. These loops could interfere with primers
Now my mentor told me that the binding of primers and hairpins have a competitive behaviour.
Can somebody explain this to me?
My thoughts are that the energy needed for the bindings are in favor for the primers for binding than for the own DNA, since bending the DNA will also costs energy.
Last edited by YoBie; 08-12-2010 at 09:10 AM.
Re: Competition between haripin folding and primers binding of DNA in a (q)PCR
|binding , competition , dna , qpcr|
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