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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Mutation to Increase Plasmid Copy Number Question

Mutation to Increase Plasmid Copy Number Question - DNA Forum

Mutation to Increase Plasmid Copy Number Question - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 06-27-2010, 06:06 PM
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Default Mutation to Increase Plasmid Copy Number Question



Hey Everyone,
I have a pSMPUW vector that is low copy number. ORI seems to be ColE1 derivative with point mutation in ROM/ROP. I have compared the ORI to the High Copy Number pUC ORI in the PLVX vector and the only difference between the two is a three base pair deletion of TGG in PLVX.

pSMPUW- GTAGCGGTGGTTTTT
PLVX- GTAGCGGTTTTT

Does anyone know if I do mutagenesis to delete the TGG nucleotides if it will create the same high copy number of the PLVX plasmid?

Thanks everyone!

Last edited by rbb147; 06-27-2010 at 06:10 PM.
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