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Mutation to Increase Plasmid Copy Number Question
I have a pSMPUW vector that is low copy number. ORI seems to be ColE1 derivative with point mutation in ROM/ROP. I have compared the ORI to the High Copy Number pUC ORI in the PLVX vector and the only difference between the two is a three base pair deletion of TGG in PLVX.
Does anyone know if I do mutagenesis to delete the TGG nucleotides if it will create the same high copy number of the PLVX plasmid?
Last edited by rbb147; 06-27-2010 at 06:10 PM.
|copy , increase , mutation , number , plasmid , question|
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