| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
06-27-2010, 06:06 PM
| |||||||||||
| |||||||||||
 Mutation to Increase Plasmid Copy Number Question Hey Everyone, I have a pSMPUW vector that is low copy number. ORI seems to be ColE1 derivative with point mutation in ROM/ROP. I have compared the ORI to the High Copy Number pUC ORI in the PLVX vector and the only difference between the two is a three base pair deletion of TGG in PLVX. pSMPUW- GTAGCGGTGGTTTTT PLVX- GTAGCGGTTTTT Does anyone know if I do mutagenesis to delete the TGG nucleotides if it will create the same high copy number of the PLVX plasmid? Thanks everyone! Last edited by rbb147; 06-27-2010 at 06:10 PM.  |
|
| Tags |
| copy , increase , mutation , number , plasmid , question |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| 2-ΔΔCT interpretation of gene copy number | piano6179 | Real-Time PCR and Quantitative PCR Forum | 2 | 04-30-2009 10:08 AM |
| YEp as a multi copy plasmid | DB_Roland | Yeast Forum | 0 | 11-27-2008 12:44 PM |
| Question about pBR322 and pUC | Liansen Liu | Protocols and Methods Forum | 0 | 07-09-2007 04:51 PM |
| Is Time The Imaginary Number? | stone | Physics Forum | 15 | 02-06-2006 04:05 PM |
| Question about number of genes leading to albinism | Ramin Yadegari | Arabidopsis and Plant Biology | 0 | 05-20-2005 05:08 PM |
Linear Mode
