| | Re: Realtime PCR
Essentially you need to amplify the DNA at different concentrations.
you use 5µl of the end solution I'm guessing.
You would seeminglu need to make serial dilutions.
Nanomolar nM 10^-9 molar 0.000,000,00X
Picomolar pM 10^-12 molar 0.000,000,000,00x (commas to help counting dont actually put them in!)
10 pM =0.000,000,000,010 M
0.1 nM=0.000,000,000,1 M
essentially convert the nanoMoles into picoMoles
0.1nM = 100 pM
10pM = 10pM ;-)
So you need a dilution series of 10 and 100pM. I would need to know what you started with to give exact examples but if you had 100pM/0.1nM solution and you need to make 10pM solution you'd do a 1 in ten. Get 45 units of buffer and add 5 units of your 100pM solution. This would dilute it by a factor of 10. (5+45=50, 5 is 10% of 50)
The units depend on what you're working with... ml, µl or whatever.
Hope that helps.