Originally Posted by boson8
thanks again for the info.
I was also wondering what other buffer are commonly used to dissolve DNA (plasmids) in other than Buffer EB which is what I am using currently.
EDTA is something I want to avoid since it is known to inhibit downstream enzymatic or sequencing reactions which is essential for my experimental analysis.
angain any advice would be really helpful.
Under Qiagen FAQ page you can see that they commonly use TE and STE. You can just leave out the EDTA.
Tris (TE without the EDTA).
Also STE (100mM NaCl, 10mM Tris-Cl, 0.1mM EDTA) leave out the EDTA as desired.
BTW, if you can keep the low 0.1mM EDTA it will benefit your storage, as it binds metal cofactors (Mg++) which reduces/stops a lot of enzyme activity, like DNAse (which can come from the cell prep, your skin, lab).
The low EDTA in the small volume of storage buffer, is usually diluted out and won't affect typical PCR/sequencing reaction/restrictions digests.
Hell you can even use spin columns (GE-healthcare G25 microspin columns) to buffer exchange the EDTA out before your downstream application (its about 5-8minute procedure).