| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-11-2009, 08:15 PM
| |||||||||||
| |||||||||||
 PCR with 1 Primer I'm trying to amplify an unsequenced region of DNA containing highly repetitive and highly polymorphic genes. I have obtained some good clean sequence but want to expand on this. Does anyone know if it would be possible to obtain small amounts of amplicon if the PCR reaction was done using only one primer? I realise you wouldn't get the exponential growth in product, and the amplicons would be of varying lengths, but if the process was repeated several times and the products purified could you end up with something that could be sequenced?  |
|
| Tags |
| pcr , primer |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Second Guessing myself on primer concentrations | nebulus | PCR - Polymerase Chain Reaction Forum | 1 | 05-01-2010 07:49 PM |
| Bad primer design ? How can I know ? | surt | Bioinformatics | 4 | 10-07-2009 07:24 AM |
| Help in calculating Tm and GC content of primer | shrinivas Dengeti | Microbiology Forum | 0 | 09-12-2009 07:09 PM |
| I need immediate help!! How to PCR primer designing? :help: | veniesh | PCR - Polymerase Chain Reaction Forum | 2 | 08-20-2009 04:00 PM |
| please help me in primer dimer | saly | Real-Time PCR and Quantitative PCR Forum | 0 | 09-06-2008 02:18 PM |
Linear Mode
