| | Re: optmizing PCR
Factors that affect PCR reactions include the concentration of MgCl2 and Taq DNA polymerase, type of Taq DNA polymerase, primer GC ratio, primer Tm, concentration of 10x reaction buffer and primer, and PCR reaction time. As mentioned, the PCR reaction can be changed by various conditions. Therefore, when amplifying a specific gene for the first time, the best PCR conditions can be found by a process of performing PCR many times and modifying the PCR conditions numerous times. Only a few conditions, from the listed conditions above, can be adjusted, such as DNA polymerase type, concentration of MgCl2 and primer, appropriate primer synthesis, and PCR reaction time. However, in order to find out the appropriate PCR conditions, PCR should be performed many times. In other words, a better solution is needed to find the best PCR conditions without having to perform PCR numerous times. To this end, iNtRON has developed the PCR Starter Kit (MS-2 Type), which provides various PCR components in a vacuum-dried premix type.
The PCR Starter Kit (MS-2 Type) consists of 32 different PCR components and will provide 32 different PCR results by one-time PCR so that the customer can control and change the PCR conditions. This new concept product, which overcomes the existing idea that PCR premixes cannot be controlled, provides the desired result in a short time by testing under various conditions, because laborious PCR set-up, in which all buffers have to be mixed individually in order to change the PCR condition, is not required. Also, iNtRONís essential technology, the vacuum-dried premix type, maximizes the productís stability and convenience in use.