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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


DNA extraction

DNA extraction - DNA Forum

DNA extraction - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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  #1  
Old 05-19-2009, 08:24 AM
Pipette Filler
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Default DNA extraction



hi all..

is it possible to extract DNA from human blood using trizol reagent?
i've been trying dis few times, but there were no visible band when i viewed the agarose gel under UV light.

im really freaking out here.


surani
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Old 07-24-2009, 08:43 AM
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Default Re: DNA extraction

I have been tying too and i have given up because its not working totally but i have been successful to do phenol extraction on the aqueous phase
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Old 08-11-2009, 02:24 PM
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Default Re: DNA extraction

Have you been using the original TRI Reagent, or TRI Reagent BD?
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Old 10-27-2009, 10:07 AM
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Default Re: DNA extraction

hi hope u r doing well .. iam new here : its successfull extracting DNA using chloroform or Guanidine

Last edited by yossra; 11-23-2009 at 05:32 PM.
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Old 07-23-2010, 07:14 PM
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Default Re: DNA extraction

Hi surani,

You should try lysing red cells and pelleting the white ones before you add trizol. Here's a recipe for a lysing solution:

10X sol (100 ml)
Potassium bicarbonate 1 g
Ammonium chloride 8.26 g
Disodic EDTA 0.03 g
Dist. H2O

Autoclave it to sterilize. Light sensitive. 10X sol is stable for a month. Work solution is stable for a day.

We use this solution to test for cell viability, so it should work as well for DNA extraction.
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Old 07-31-2010, 10:59 AM
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Default Re: DNA extraction

thanx guys..

but i already switch to Qiagen kit. the yield are good now.
thanx again.

cheers
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Old 11-04-2010, 07:59 PM
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Default Re: DNA extraction

try Favorgen is as good as Qgen and cheaper
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Old 11-22-2010, 10:15 PM
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Default Re: DNA extraction

exactly
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Old 12-12-2010, 06:23 PM
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Default Re: DNA extraction

I SUGGEST TO USE qiagene kit it is excellent and the quality and quantity of extraced DNA is high . Also, its procedure is easy and simple
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