DNA extraction

[surani]

hi all..

is it possible to extract DNA from human blood using trizol reagent?
i've been trying dis few times, but there were no visible band when i viewed the agarose gel under UV light.

im really freaking out here.

surani

[WAMBUI]

I have been tying too and i have given up because its not working totally but i have been successful to do phenol extraction on the aqueous phase

[evolve]

Have you been using the original TRI Reagent, or TRI Reagent BD?

[yossra]

hi hope u r doing well .. iam new here : its successfull extracting DNA using chloroform or Guanidine

[luisillo]

Hi surani,

You should try lysing red cells and pelleting the white ones before you add trizol. Here's a recipe for a lysing solution:

10X sol (100 ml)
Potassium bicarbonate 1 g
Ammonium chloride 8.26 g
Disodic EDTA 0.03 g
Dist. H2O

Autoclave it to sterilize. Light sensitive. 10X sol is stable for a month. Work solution is stable for a day.

We use this solution to test for cell viability, so it should work as well for DNA extraction.

[surani]

thanx guys..

but i already switch to Qiagen kit. the yield are good now.
thanx again.

cheers

[MEDIBENA]

try Favorgen is as good as Qgen and cheaper

[papay13]

exactly

[smgh62]

I SUGGEST TO USE qiagene kit it is excellent and the quality and quantity of extraced DNA is high . Also, its procedure is easy and simple