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surani 05-19-2009 08:24 AM

DNA extraction
 
hi all..

is it possible to extract DNA from human blood using trizol reagent?
i've been trying dis few times, but there were no visible band when i viewed the agarose gel under UV light.

im really freaking out here.


surani

WAMBUI 07-24-2009 08:43 AM

Re: DNA extraction
 
I have been tying too and i have given up because its not working totally but i have been successful to do phenol extraction on the aqueous phase

evolve 08-11-2009 02:24 PM

Re: DNA extraction
 
Have you been using the original TRI Reagent, or TRI Reagent BD?

yossra 10-27-2009 10:07 AM

Re: DNA extraction
 
hi hope u r doing well .. iam new here : its successfull extracting DNA using chloroform or Guanidine

luisillo 07-23-2010 07:14 PM

Re: DNA extraction
 
Hi surani,

You should try lysing red cells and pelleting the white ones before you add trizol. Here's a recipe for a lysing solution:

10X sol (100 ml)
Potassium bicarbonate 1 g
Ammonium chloride 8.26 g
Disodic EDTA 0.03 g
Dist. H2O

Autoclave it to sterilize. Light sensitive. 10X sol is stable for a month. Work solution is stable for a day.

We use this solution to test for cell viability, so it should work as well for DNA extraction.

surani 07-31-2010 10:59 AM

Re: DNA extraction
 
thanx guys..

but i already switch to Qiagen kit. the yield are good now.
thanx again.

cheers :)

MEDIBENA 11-04-2010 07:59 PM

Re: DNA extraction
 
try Favorgen is as good as Qgen and cheaper :)

papay13 11-22-2010 10:15 PM

Re: DNA extraction
 
exactly :)

smgh62 12-12-2010 06:23 PM

Re: DNA extraction
 
I SUGGEST TO USE qiagene kit it is excellent and the quality and quantity of extraced DNA is high . Also, its procedure is easy and simple


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