Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (
-   DNA Forum (
-   -   DNA extraction (

surani 05-19-2009 08:24 AM

DNA extraction
hi all..

is it possible to extract DNA from human blood using trizol reagent?
i've been trying dis few times, but there were no visible band when i viewed the agarose gel under UV light.

im really freaking out here.


WAMBUI 07-24-2009 08:43 AM

Re: DNA extraction
I have been tying too and i have given up because its not working totally but i have been successful to do phenol extraction on the aqueous phase

evolve 08-11-2009 02:24 PM

Re: DNA extraction
Have you been using the original TRI Reagent, or TRI Reagent BD?

yossra 10-27-2009 10:07 AM

Re: DNA extraction
hi hope u r doing well .. iam new here : its successfull extracting DNA using chloroform or Guanidine

luisillo 07-23-2010 07:14 PM

Re: DNA extraction
Hi surani,

You should try lysing red cells and pelleting the white ones before you add trizol. Here's a recipe for a lysing solution:

10X sol (100 ml)
Potassium bicarbonate 1 g
Ammonium chloride 8.26 g
Disodic EDTA 0.03 g
Dist. H2O

Autoclave it to sterilize. Light sensitive. 10X sol is stable for a month. Work solution is stable for a day.

We use this solution to test for cell viability, so it should work as well for DNA extraction.

surani 07-31-2010 10:59 AM

Re: DNA extraction
thanx guys..

but i already switch to Qiagen kit. the yield are good now.
thanx again.

cheers :)

MEDIBENA 11-04-2010 07:59 PM

Re: DNA extraction
try Favorgen is as good as Qgen and cheaper :)

papay13 11-22-2010 10:15 PM

Re: DNA extraction
exactly :)

smgh62 12-12-2010 06:23 PM

Re: DNA extraction
I SUGGEST TO USE qiagene kit it is excellent and the quality and quantity of extraced DNA is high . Also, its procedure is easy and simple

All times are GMT. The time now is 10:02 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09527 seconds with 11 queries