DNA extraction
 

hi all..

is it possible to extract DNA from human blood using trizol reagent?
i've been trying dis few times, but there were no visible band when i viewed the agarose gel under UV light.

im really freaking out here.

surani

Re: DNA extraction
 

I have been tying too and i have given up because its not working totally but i have been successful to do phenol extraction on the aqueous phase

Re: DNA extraction
 

Have you been using the original TRI Reagent, or TRI Reagent BD?

Re: DNA extraction
 

hi hope u r doing well .. iam new here : its successfull extracting DNA using chloroform or Guanidine

Re: DNA extraction
 

Hi surani,

You should try lysing red cells and pelleting the white ones before you add trizol. Here's a recipe for a lysing solution:

10X sol (100 ml)
Potassium bicarbonate 1 g
Ammonium chloride 8.26 g
Disodic EDTA 0.03 g
Dist. H2O

Autoclave it to sterilize. Light sensitive. 10X sol is stable for a month. Work solution is stable for a day.

We use this solution to test for cell viability, so it should work as well for DNA extraction.

Re: DNA extraction
 

thanx guys..

but i already switch to Qiagen kit. the yield are good now.
thanx again.

cheers :)

Re: DNA extraction
 

try Favorgen is as good as Qgen and cheaper :)

Re: DNA extraction
 

exactly :)

Re: DNA extraction
 

I SUGGEST TO USE qiagene kit it is excellent and the quality and quantity of extraced DNA is high . Also, its procedure is easy and simple