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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Gateway cloning in BACS

Gateway cloning in BACS - DNA Forum

Gateway cloning in BACS - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 04-16-2009, 01:18 PM
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Default Gateway cloning in BACS



Hi.

We want to insert a gateway flanked positive/negative cassette into a BAC (by recombineering) and then replace it with a series of sequences (eg cDNA´s) in a gateway reaction. Does anyone know if this is feasible? any references?
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