Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


competent cell,cloning vector, transformation

competent cell,cloning vector, transformation - DNA Forum

competent cell,cloning vector, transformation - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-08-2009, 03:32 AM
anxenz88's Avatar
Pipette Filler
Points: 14, Level: 1 Points: 14, Level: 1 Points: 14, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Cool competent cell,cloning vector, transformation



Did someone know how the CaCl2 can make the membrane cell become permeable to DNA in transformation?
Reply With Quote
  #2  
Old 03-16-2009, 07:34 PM
Pipette Filler
Points: 3,764, Level: 40 Points: 3,764, Level: 40 Points: 3,764, Level: 40
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2008
Posts: 27
Thanks: 0
Thanked 2 Times in 1 Post
Default Re: competent cell,cloning vector, transformation

maybe this link will hilp

[Only registered users see links. ]
Reply With Quote
The Following 2 Users Say Thank You to osama al refa'e For This Useful Post:
pigfarmer (07-07-2009), rishabhindore (11-04-2009)
  #3  
Old 03-20-2009, 01:33 AM
aftabac's Avatar
Nobel Laureate
 
Join Date: Jun 2006
Location: Pakistan
Posts: 570
Thanks: 5
Thanked 41 Times in 34 Posts
Default Re: competent cell,cloning vector, transformation

Hi
its nice link with lot of more informations on different topics.....

regards
aftab
Reply With Quote
  #4  
Old 05-19-2009, 05:01 PM
Pipette Filler
Points: 949, Level: 17 Points: 949, Level: 17 Points: 949, Level: 17
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2008
Location: pakistan
Posts: 24
Thanks: 5
Thanked 0 Times in 0 Posts
Default Re: competent cell,cloning vector, transformation

Hi,

for transformation we make competent cells to enable them for uptake of foreign DNA.For this purpose we use different methods of which one is chemical method i.e using CaCl2 which gives shocks to membrane of the cells rendering them fragile thus helping in the uptake of foreign genome.
Reply With Quote
  #5  
Old 07-06-2009, 07:04 AM
Pipette Filler
Points: 74, Level: 1 Points: 74, Level: 1 Points: 74, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2009
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: competent cell,cloning vector, transformation

Hi Obama, the link posted by you is not opening, can you check it again?
Reply With Quote
  #6  
Old 07-07-2009, 05:44 PM
Pipette Filler
Points: 3,764, Level: 40 Points: 3,764, Level: 40 Points: 3,764, Level: 40
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2008
Posts: 27
Thanks: 0
Thanked 2 Times in 1 Post
Default Re: competent cell,cloning vector, transformation

hi
Obama , hh, nice one , dear liquid nitrogen
i checked the link more than one time and it worked with me
let me know if it work with you ok?>>> have a nice day
Reply With Quote
  #7  
Old 08-30-2009, 02:52 PM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: competent cell,cloning vector, transformation

Hi, maybe someone of you could help me to understand the reason of my problem. I want to clone 2 genes of heme proteins, so I added the second gene to the first one, present on the plasmid. I didn't remove the stop codon of the first gene because i put the ribosome binding site before the second one. In this way I would like to have 2 separated proteins. As heme proteins are transfered to periplasm I don't need any tag. I checked also the presence of right sequences in the plasmid amplificated by PCR. SDS-PAGE of bacteries growth showed the right size of the proteins which should be red but they are white. Did somebody do something like this?
Reply With Quote
Reply

Tags
cell , cloning , competent , transformation , vector


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Yeast Competent Cells dnamolecule Yeast Forum 1 09-07-2008 05:50 PM
Competent Cells Using CaCl2 gandalfthegrey Microbiology Forum 2 10-28-2007 08:05 AM
competent cells aftabac Protocols and Methods Forum 0 03-15-2007 03:57 PM
Is there any difference between BL21 and BL21(DE3) competent cells? Teh Yufen Protocols and Methods Forum 3 07-28-2006 08:39 AM
Protocol for making competent cell Michael Sullivan Protocols and Methods Forum 0 05-05-2006 03:07 PM


All times are GMT. The time now is 03:40 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14790 seconds with 16 queries