I was able to extract DNA from bone of a turtle species and see a week DNA band comparison to muscle tissue when I run the DNA products on the gel. I used Kalmar et al.,2000 protocol;
- Extraction buffer (0.1 M EDTA, 0.5% N-laurylsarcosinate, 100 mg/ml Proteinase K)
- 3.5 µl (1 µg/µl) Dextran Blue
- 250 µl 4 M NH4-acetate
- 500 µl EtOH
- 25 µl dH2O
I performed PCR of mtDNA gene(app. 850bp) to be optimized previously. But I didn’t have amplification. I used in amplification;
- 0.6 µl Primer F and R
- 2 µl dNTP (2.5 mM)
- 1.5 µl Bovin Serum Albumine (BSA) (4 µg/ml)
- 0.4 µl Taq poly.
- 6 µl DNA (uncertain DNA quantitative)
I will be happy if you help me for both PCR and DNA extraction.