|Register||Search||Today's Posts||Mark Forums Read|
|DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.|
| ||LinkBack||Thread Tools||Display Modes|
Confusion with Sense and Antisense....
Just a quick question as I am totally boogled....
I know that the antisense strand is the strand that results in the production of a protein. Then a mutation is not as significant if it occurs on this strand?
I am confused as I have cloned my gene of interest into a vector and upon sequencing I find a mutation using the reverse primer ( I have ordered the forward primer and shall sequence in this dircetion as well).
Can someone clear this up for me...please
Last edited by admin; 01-09-2009 at 01:09 AM.
Re: Confusion with Sense and Antisense....
The sense strand is the strand that makes a protein. But sense or antisense, DNA is made of 2 complementary anti parallel strands. Now if there is a mutation on one of the strands you will see a mutation with the complementary base on the other. THEREFORE both DNA strands will then be mutated.
|antisense , confusion , sense|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Sense and antisense in RNA interference.?||ana||RNAi and SiRNA Forum||2||09-02-2009 03:40 AM|
|Sense probes ISH||Els||Zebrafish Forum||1||12-29-2008 01:05 PM|
|Role of Antisence RNA||aftabac||General Science Questions and Layperson Board||1||11-25-2006 08:07 PM|