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Confusion with Sense and Antisense....

Confusion with Sense and Antisense.... - DNA Forum

Confusion with Sense and Antisense.... - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 01-06-2009, 09:41 AM
cor cor is offline
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Default Confusion with Sense and Antisense....



Just a quick question as I am totally boogled....

I know that the antisense strand is the strand that results in the production of a protein. Then a mutation is not as significant if it occurs on this strand?

I am confused as I have cloned my gene of interest into a vector and upon sequencing I find a mutation using the reverse primer ( I have ordered the forward primer and shall sequence in this dircetion as well).


Can someone clear this up for me...please

Last edited by admin; 01-09-2009 at 02:09 AM.
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Old 01-09-2009, 02:08 AM
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Default Re: Confusion with Sense and Antisense....

Hey Cor
The sense strand is the strand that makes a protein. But sense or antisense, DNA is made of 2 complementary anti parallel strands. Now if there is a mutation on one of the strands you will see a mutation with the complementary base on the other. THEREFORE both DNA strands will then be mutated.
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