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| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
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#1
01-06-2009, 08:41 AM
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 Confusion with Sense and Antisense.... Just a quick question as I am totally boogled....  I know that the antisense strand is the strand that results in the production of a protein. Then a mutation is not as significant if it occurs on this strand? I am confused as I have cloned my gene of interest into a vector and upon sequencing I find a mutation using the reverse primer ( I have ordered the forward primer and shall sequence in this dircetion as well). Can someone clear this up for me...please Last edited by admin; 01-09-2009 at 01:09 AM.  |
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#2
01-09-2009, 01:08 AM
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| Re: Confusion with Sense and Antisense.... Hey Cor The sense strand is the strand that makes a protein. But sense or antisense, DNA is made of 2 complementary anti parallel strands. Now if there is a mutation on one of the strands you will see a mutation with the complementary base on the other. THEREFORE both DNA strands will then be mutated. |
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| antisense , confusion , sense |
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