Recently I've doing DNA extraction from mouse tail. But there are two steps in the protocol that i would like to clarify. During the precipitation of the protein and other debrises of the cell using the NaCl, what will happpen if we happen to vorteks too much of the eppendorf tube? I happened to vorteks the tube for 15 min. After that realising maybe that would be not enough to mix the Nacl with the content in the tube i vorteks again for around 5 minutes more, does this has any effect on the DNA that I will be getting? Please do advice.
On the last step in the protocol, the DNA pellet need to be resuspended in DNAse & RNAse free water. Does the word resuspended mean mix the DNA pellet with the water or just put in water into the tube without mixing using the pipet?Please do advice?
Today I'll be doing the PCR, just worried whether i have extracted the DNA or not on the previous day.