SOuthern blot analysis nonradioactive probes

Amezua · #1 10-02-2008, 02:53 PM

Hi everybody,

I got to do a Southern blot analysis using as probes biotin labeled oligos. Does any of you have a good protocol about how to use nonradioactive probes for a Southern blot analysis?

Thanks a lot
Pilar

molio · #2 10-05-2008, 05:30 PM

Hi
You could use DIG-labeled DNA probes.
Using the DIG-Easy-Hyb from Roche.

Let us know how it goes

Amezua · #3 10-13-2008, 03:42 PM

Hi thanks for your answer

I would like to use the probes as positive control for the Southern blot. Do you know how it is possible to clon them?

Thanks again
Pilar

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SOuthern blot analysis nonradioactive probes

SOuthern blot analysis nonradioactive probes - DNA Forum

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