Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


SOuthern blot analysis nonradioactive probes

SOuthern blot analysis nonradioactive probes - DNA Forum

SOuthern blot analysis nonradioactive probes - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-02-2008, 02:53 PM
Pipette Filler
Points: 264, Level: 5 Points: 264, Level: 5 Points: 264, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default SOuthern blot analysis nonradioactive probes



Hi everybody,

I got to do a Southern blot analysis using as probes biotin labeled oligos. Does any of you have a good protocol about how to use nonradioactive probes for a Southern blot analysis?

Thanks a lot
Pilar
Reply With Quote
  #2  
Old 10-05-2008, 05:30 PM
Pipette Filler
Points: 225, Level: 4 Points: 225, Level: 4 Points: 225, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: SOuthern blot analysis nonradioactive probes

Hi
You could use DIG-labeled DNA probes.
Using the DIG-Easy-Hyb from Roche.

Let us know how it goes
Reply With Quote
  #3  
Old 10-13-2008, 03:42 PM
Pipette Filler
Points: 264, Level: 5 Points: 264, Level: 5 Points: 264, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: SOuthern blot analysis nonradioactive probes

Hi thanks for your answer

I would like to use the probes as positive control for the Southern blot. Do you know how it is possible to clon them?

Thanks again
Pilar
Reply With Quote
Reply

Tags
analysis , blot , nonradioactive , probes , southern


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Non-Radioactive Southern Blot Probes fatima DNA Techniques 5 10-02-2008 03:13 PM
Problems doing Northern Blot with small probes (oligo, riboprobe,PCR and random primed) Sharon Protocols and Methods Forum 1 02-12-2008 08:44 AM
southern blot question phillipw Knockout Mouse Forum 3 01-29-2008 10:37 AM


All times are GMT. The time now is 03:45 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11997 seconds with 16 queries