| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
10-24-2008, 03:14 PM
| |||||||||||
| |||||||||||
 GTC Buffer for sample resuspension HELP!!! I have a protocol for resuspending dried bacterial sample after grinding in liquid nitrogen. It says to use GTC buffer. Does anyone have a protocol for this?? I can't find one. Thanks  |
|
#2
11-14-2008, 02:45 PM
| |||||||||||
| |||||||||||
 Re: GTC Buffer for sample resuspension Might be too late, but if you are still looking for it: GTC is guanidine thiocyanate. Buffers for lysing cells differ, but look, in general, like this: 40 mM Tris-HCl, pH 8.0 4 M guanidine thiocyanate 20 mM Na acetate 1% N-lauroylsarcosine Na salt 1% b-ME (add fresh) Good luck! |
| The Following User Says Thank You to TerAle For This Useful Post: | ||
shanti32 (11-25-2008)
| ||
|
| Tags |
| buffer , gtc , resuspension , sample |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| protein purification | magnificientleo | Proteomics Forum | 11 | 12-29-2010 08:01 AM |
| SDS-PAGE Protocol | moleculardude | Protein Science | 14 | 02-12-2010 10:27 AM |
| Production of Monoclonal Antibodies - Technique in Mouse | Cellbiogal | Antibody Forum | 2 | 01-08-2009 09:52 PM |
| Methods Digest, Vol 18, Issue 19 1. Qiagen buffer PN | Jess | Protocols and Methods Forum | 0 | 11-22-2006 09:45 PM |
| Qiagen buffer PN | Daniel Ciznadija | Protocols and Methods Forum | 0 | 11-19-2006 09:12 PM |
Linear Mode
