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GTC Buffer for sample resuspension

GTC Buffer for sample resuspension - DNA Forum

GTC Buffer for sample resuspension - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 10-24-2008, 03:14 PM
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Question GTC Buffer for sample resuspension



HELP!!! I have a protocol for resuspending dried bacterial sample after grinding in liquid nitrogen. It says to use GTC buffer. Does anyone have a protocol for this?? I can't find one. Thanks
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Old 11-14-2008, 03:45 PM
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Default Re: GTC Buffer for sample resuspension

Might be too late, but if you are still looking for it: GTC is guanidine thiocyanate.

Buffers for lysing cells differ, but look, in general, like this:
40 mM Tris-HCl, pH 8.0
4 M guanidine thiocyanate
20 mM Na acetate
1% N-lauroylsarcosine Na salt
1% b-ME (add fresh)

Good luck!
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