| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#2
03-30-2007, 06:18 AM
| |||||||||||
| |||||||||||
 Yes. It can be. Electrophoresis run in high heat remember? So all the intercellular bonds in DNa will be broken. 1 RE or 2 RE may be helpful to break down several fragment, to see the result easier. DNA ladder will be needed as reference.  |
|
#3
03-30-2007, 02:37 PM
| |||||||||||
| |||||||||||
| Re: Can the size of a supercoiled plasmid DNA be No, you cannot determine the size of a supercoiled plasmid by standard electrophoresis. You must first linearize the plasmid with a restriction enzyme to remove the supercoils, then you can determine size by comparing to a standard ladder. Supercoiled plasmid runs at an unpredictably smaller molecular weight than linearized plasmid. |
|
| Tags |
| dna , plasmid , size , supercoiled |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| How can we justify the appearance of supercoiled plasmid | Bhargav S | DNA Techniques | 4 | 10-13-2011 11:24 PM |
| What is the size of the plasmid DNA pCMV-GFP? | andromedia | DNA Techniques | 3 | 06-02-2010 02:19 PM |
| supercoiled plasmid DNA | jahaley | DNA Techniques | 3 | 01-16-2008 05:07 PM |
| GNU units and units.dat; Units of Measurement and Unit Conversion | James Redford | Physics Forum | 0 | 07-31-2005 12:08 PM |
| yeast plasmid preps | johnspar@earthlink.net | Yeast Forum | 0 | 02-20-2004 01:13 AM |
Linear Mode
