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Can the size of a supercoiled plasmid DNA be

Can the size of a supercoiled plasmid DNA be - DNA Forum

Can the size of a supercoiled plasmid DNA be - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 03-30-2007, 04:24 AM
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Default Can the size of a supercoiled plasmid DNA be



determined by using standard DNA fragments electrophoresis ? in parallel?
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Old 03-30-2007, 06:18 AM
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Yes. It can be. Electrophoresis run in high heat remember? So all the intercellular bonds in DNa will be broken.

1 RE or 2 RE may be helpful to break down several fragment, to see the result easier.

DNA ladder will be needed as reference.
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Old 03-30-2007, 02:37 PM
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Default Re: Can the size of a supercoiled plasmid DNA be

No, you cannot determine the size of a supercoiled plasmid by standard electrophoresis.

You must first linearize the plasmid with a restriction enzyme to remove the supercoils, then you can determine size by comparing to a standard ladder.

Supercoiled plasmid runs at an unpredictably smaller molecular weight than linearized plasmid.
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