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|DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.|
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Can the size of a supercoiled plasmid DNA be
determined by using standard DNA fragments electrophoresis ? in parallel?
Yes. It can be. Electrophoresis run in high heat remember? So all the intercellular bonds in DNa will be broken.
1 RE or 2 RE may be helpful to break down several fragment, to see the result easier.
DNA ladder will be needed as reference.
Re: Can the size of a supercoiled plasmid DNA be
No, you cannot determine the size of a supercoiled plasmid by standard electrophoresis.
You must first linearize the plasmid with a restriction enzyme to remove the supercoils, then you can determine size by comparing to a standard ladder.
Supercoiled plasmid runs at an unpredictably smaller molecular weight than linearized plasmid.
|dna , plasmid , size , supercoiled|
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