Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   DNA Forum (http://www.molecularstation.com/forum/dna-forum/)
-   -   Can the size of a supercoiled plasmid DNA be (http://www.molecularstation.com/forum/dna-forum/1329-can-size-supercoiled-plasmid-dna.html)

shan1946 03-30-2007 04:24 AM

Can the size of a supercoiled plasmid DNA be
 
determined by using standard DNA fragments electrophoresis ? in parallel?

hopefully Zen 03-30-2007 06:18 AM

Yes. It can be. Electrophoresis run in high heat remember? So all the intercellular bonds in DNa will be broken.

1 RE or 2 RE may be helpful to break down several fragment, to see the result easier.

DNA ladder will be needed as reference.

kmunson779 03-30-2007 02:37 PM

Re: Can the size of a supercoiled plasmid DNA be
 
No, you cannot determine the size of a supercoiled plasmid by standard electrophoresis.

You must first linearize the plasmid with a restriction enzyme to remove the supercoils, then you can determine size by comparing to a standard ladder.

Supercoiled plasmid runs at an unpredictably smaller molecular weight than linearized plasmid.


All times are GMT. The time now is 01:05 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08977 seconds with 11 queries