Can the size of a supercoiled plasmid DNA be
determined by using standard DNA fragments electrophoresis ? in parallel?
Yes. It can be. Electrophoresis run in high heat remember? So all the intercellular bonds in DNa will be broken.
1 RE or 2 RE may be helpful to break down several fragment, to see the result easier.
DNA ladder will be needed as reference.
Re: Can the size of a supercoiled plasmid DNA be
No, you cannot determine the size of a supercoiled plasmid by standard electrophoresis.
You must first linearize the plasmid with a restriction enzyme to remove the supercoils, then you can determine size by comparing to a standard ladder.
Supercoiled plasmid runs at an unpredictably smaller molecular weight than linearized plasmid.
|All times are GMT. The time now is 11:55 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved