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| DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function. |
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| Purpose of dephosphorylating a DNA backbone? |
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| Using a phosphatase chops off terminal phosphate groups from a DNA strand which are then unable to join up again (ligate). Technique is used to isolate specific strands after using a restriction enzyme, for example. Or to prevent plasmids re-circularising. |
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| backbone , dephosphorylating , dna , purpose |
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