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DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Purpose of dephosphorylating a DNA backbone?

Purpose of dephosphorylating a DNA backbone? - DNA Forum

Purpose of dephosphorylating a DNA backbone? - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


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Old 03-27-2007, 01:59 AM
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Default Purpose of dephosphorylating a DNA backbone?



Purpose of dephosphorylating a DNA backbone?
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Old 03-27-2007, 03:03 AM
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Using a phosphatase chops off terminal phosphate groups from a DNA strand which are then unable to join up again (ligate).
Technique is used to isolate specific strands after using a restriction enzyme, for example. Or to prevent plasmids re-circularising.
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