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-   -   Purpose of dephosphorylating a DNA backbone? (http://www.molecularstation.com/forum/dna-forum/1263-purpose-dephosphorylating-dna-backbone.html)

starla_o0 03-27-2007 01:59 AM

Purpose of dephosphorylating a DNA backbone?
 
Purpose of dephosphorylating a DNA backbone?

bumperbuffer 03-27-2007 03:03 AM

Using a phosphatase chops off terminal phosphate groups from a DNA strand which are then unable to join up again (ligate).
Technique is used to isolate specific strands after using a restriction enzyme, for example. Or to prevent plasmids re-circularising.


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