Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > DNA Forum
Register Search Today's Posts Mark Forums Read

DNA Forum Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


How does a real DNA or RNA probe "stick" to the...

How does a real DNA or RNA probe "stick" to the... - DNA Forum

How does a real DNA or RNA probe "stick" to the... - Discuss DNA, the molecule of hereditary. Topics include DNA structure, DNA replication, DNA function.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-25-2007, 08:07 AM
Pipette Filler
Points: 1,351, Level: 21 Points: 1,351, Level: 21 Points: 1,351, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default How does a real DNA or RNA probe "stick" to the...



...target DNA sequence in DNA fingerprinting?
Reply With Quote
  #2  
Old 03-25-2007, 09:01 AM
Pipette Filler
Points: 1,336, Level: 21 Points: 1,336, Level: 21 Points: 1,336, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default

DNA/RNA probes will stick to their respective DNA/RNA sequence when the two sequences are aligned to each other so they can form hydrogen bonds. For instance, a given probe ATTCAGGTC with locate the DNA sequence TAAGTCCAG and form 2 or 3 hydrogen bonds with the opposing nucleotide based on which complex it is. I believe the A/T complex has three hydrogen bonds and the C/G complex has 2 hydrogen bonds. Therefore, in our example probe we will find 23 hydrogen bonds, which gives the probe its stickiness.
Reply With Quote
Reply

Tags
dna , probe , real , rna , stick


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Real Time-PCR probe design Ganto Real-Time PCR and Quantitative PCR Forum 1 03-22-2010 08:00 PM
Real Time-PCR probe design Ganto Real-Time PCR and Quantitative PCR Forum 1 09-17-2009 11:24 AM
Real time pcr multiplex with probe vs. sybrgreen Scott Brown Protocols and Methods Forum 1 05-13-2009 03:35 PM
problems with probe binding to 28S and 18S RNA only on Northerns Wendy Ingram Protocols and Methods Forum 0 09-11-2006 02:30 AM


All times are GMT. The time now is 06:13 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11539 seconds with 16 queries