I am using Trizol for extracting genomic DNA from cerebrospinal fluids. I am expecting between 2-5 ng genomic DNA from my sample but in PCR there is no amplification. I have tried several different changes in trizol extraction protocol to get a better yield but nanodrop is not sensitive enough and PCR shows no amplification. I have around 500 human samples lysed in TRIZOL waiting to be extracted but I have failed to optimize the extraction yet.
My questions are:
1. Does Trizol eluted DNA contain any PCR inhibitors? If yes, then how to clean the eluted DNA (I have around 100 samples already extracted)
2. Can I apply the TRIZOL organic phase directly on to a DNA column (DNA micro kit)?
Will be grateful for any tips.