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Proteinase K

Proteinase K - DNA Extraction Forum

Proteinase K - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 03-06-2012, 12:53 PM
Pipette Filler
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Default Proteinase K



Hello! does anyone know if proteinase K is autoclavable???? Itīs for an extraction buffer and I need it free of any other DNA!!! thanks!
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Old 03-10-2012, 05:37 AM
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Default Re: Proteinase K

Why don't you try sterilizing it by filtering with a 0.2 u filter? That way you avoid denaturation of the enzyme. If you do filter it, I recommend using low protein binding filters, so that you don't lose enzyme in the process.

Good luck!
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Old 03-10-2012, 02:12 PM
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Default Re: Proteinase K

thanks!!!!
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