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-   -   Proteinase K (http://www.molecularstation.com/forum/dna-extraction-forum/84832-proteinase-k.html)

carobiotech 03-06-2012 12:53 PM

Proteinase K
 
Hello! does anyone know if proteinase K is autoclavable???? Itīs for an extraction buffer and I need it free of any other DNA!!! thanks! :)

luisillo 03-10-2012 05:37 AM

Re: Proteinase K
 
Why don't you try sterilizing it by filtering with a 0.2 u filter? That way you avoid denaturation of the enzyme. If you do filter it, I recommend using low protein binding filters, so that you don't lose enzyme in the process.

Good luck!

carobiotech 03-10-2012 02:12 PM

Re: Proteinase K
 
thanks!!!!


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