Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques > DNA Extraction Forum
Register Search Today's Posts Mark Forums Read

DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


Clean DNA from Sticky/Dirty/PCR inhibitor Marine Inverts

Clean DNA from Sticky/Dirty/PCR inhibitor Marine Inverts - DNA Extraction Forum

Clean DNA from Sticky/Dirty/PCR inhibitor Marine Inverts - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-13-2011, 06:44 AM
jez jez is offline
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Clean DNA from Sticky/Dirty/PCR inhibitor Marine Inverts



Hello All,

I'm trying to extract some good clean DNA from a seacucumber, the seacucumber in question is a particularity slimy individual. I can get DNA (as confirmed by a gel) but this DNA contains lots of things in it that prevents my PCR working. I have tried Spin columns, PCI, Pro_k extractions, chelex, HotShot as extraction methods and all have little effect on what ever is preventing successful PCR. I have also tried diluting the DNA all the way down to 1:1000 but no use. It is not me my positive and negative controls are all as they should be.

Does anyone know of any methods, washes, enzymes etc that i could try to see if they will clean my DNA up. I know that lots of Marine Inverts also cause similar problems so I'm hoping that someone has some good tips/techniques I could try.

Any help is appreciated.

Thanks

Jez
Reply With Quote
Reply

Tags
clean , dna , inhibitor , inverts , marine , sticky or dirty or pcr


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 01:41 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10834 seconds with 15 queries