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|DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.|
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Clean DNA from Sticky/Dirty/PCR inhibitor Marine Inverts
I'm trying to extract some good clean DNA from a seacucumber, the seacucumber in question is a particularity slimy individual. I can get DNA (as confirmed by a gel) but this DNA contains lots of things in it that prevents my PCR working. I have tried Spin columns, PCI, Pro_k extractions, chelex, HotShot as extraction methods and all have little effect on what ever is preventing successful PCR. I have also tried diluting the DNA all the way down to 1:1000 but no use. It is not me my positive and negative controls are all as they should be.
Does anyone know of any methods, washes, enzymes etc that i could try to see if they will clean my DNA up. I know that lots of Marine Inverts also cause similar problems so I'm hoping that someone has some good tips/techniques I could try.
Any help is appreciated.
|clean , dna , inhibitor , inverts , marine , sticky or dirty or pcr|