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-   -   problem with plasmid isolation from DH5alpha (http://www.molecularstation.com/forum/dna-extraction-forum/76657-problem-plasmid-isolation-dh5alpha.html)

Chinnu 03-25-2011 07:32 AM

problem on plasmid isolation
 
hi,

i am facing some problem on plasmid isolation from dh5 alpha. i repeated the isolation of plasmid from the same by three times, by using the phenol-chloroform method. all the time it gives a concentration around 20ng/ul.

if anyone have some idea,please help me


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