|Register||Search||Today's Posts||Mark Forums Read|
|DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.|
| ||LinkBack||Thread Tools||Display Modes|
Best method or kit for DNA extraction from bovine sperm ??
hi, I always have problem with DNA extraction from bovine sperm , most of kits are used for animal cell and tissue don't work for sperm and I cant get a good result. in addition I have used phenol-chloroform method too, but I usually visualize smear with DNA band in agarose gel! if anyone have experience or information let me know and help me
: so what is the best method for high purity and quality DNA extraction from bovine sperm?
and anyone have idea about modifying iNtron animal cell &tissue kit(cat no#14071) for sperm?
i apologize for my bad english and im really thanksfull of anyone can help me
Re: Best method or kit for DNA extraction from bovine sperm ??
I will be using the Qiagen "DNeasy blood and tissue kit" for extraction from animal sperm. There is some "user developed protocol" on their web site you can check out. I think that for sperm cells you need to use DTT to break the disulfide bonds.
|bovine , dna , extraction , kit , method , sperm|
|Thread||Thread Starter||Forum||Replies||Last Post|
|DNA extraction from bovine semen||Nikola||DNA Extraction Forum||1||09-22-2010 03:20 PM|
|Extraction of whole blood DNA and RNA||bioteke||DNA Extraction Forum||0||07-07-2010 03:33 AM|
|DNA extraction Method from Blood||molgen||DNA Forum||4||10-26-2009 04:34 PM|
|Sperm proteome gives “tantalising glimpse” towards the origin of sex||kiki06||Proteomics Forum||0||11-13-2006 09:34 PM|