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Best method or kit for DNA extraction from bovine sperm ??

Best method or kit for DNA extraction from bovine sperm ?? - DNA Extraction Forum

Best method or kit for DNA extraction from bovine sperm ?? - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 12-28-2010, 01:28 PM
Pipette Filler
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Question Best method or kit for DNA extraction from bovine sperm ??



hi, I always have problem with DNA extraction from bovine sperm , most of kits are used for animal cell and tissue don't work for sperm and I cant get a good result. in addition I have used phenol-chloroform method too, but I usually visualize smear with DNA band in agarose gel! if anyone have experience or information let me know and help me
: so what is the best method for high purity and quality DNA extraction from bovine sperm?
and anyone have idea about modifying iNtron animal cell &tissue kit(cat no#14071) for sperm?
i apologize for my bad english and im really thanksfull of anyone can help me
best regard
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Old 01-13-2011, 01:38 PM
Pipette Filler
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Default Re: Best method or kit for DNA extraction from bovine sperm ??

Hi
I will be using the Qiagen "DNeasy blood and tissue kit" for extraction from animal sperm. There is some "user developed protocol" on their web site you can check out. I think that for sperm cells you need to use DTT to break the disulfide bonds.

Best regards.
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The Following 3 Users Say Thank You to Nikola For This Useful Post:
admin (01-13-2011), luisillo (01-14-2011), s-vet (01-14-2011)
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