hi, I always have problem with DNA extraction from bovine sperm , most of kits are used for animal cell and tissue don't work for sperm and I cant get a good result. in addition I have used phenol-chloroform method too, but I usually visualize smear with DNA band in agarose gel! if anyone have experience or information let me know and help me
: so what is the best method for high purity and quality DNA extraction from bovine sperm?
and anyone have idea about modifying iNtron animal cell &tissue kit(cat no#14071) for sperm?
i apologize for my bad english and im really thanksfull of anyone can help me