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my DNA is degrading..
Im trying to purify DNA from plasmodium parasite for souther but it keeps degrading! im incubating it with TSE, SDS and proteinase K ON.
does anybody has suggestions for how to prevent DNA from degrading during the purification???
Re: my DNA is degrading..
I would make sure you have everything on ice just in case, and possibly add a Dnase inhibitor.
Inhibitors of DNase:
You could also try isolating the nuclear protein extract that would get rid of a lot of DNases.
see this post for more:
DNAse Inhibition for Perfect DNA Isolations
Last edited by admin; 12-22-2010 at 12:35 PM.
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|degrading , dna|